Despite her assurances, Beijing has stepped up pressure on the island Online Cigarettes Store USA to seek "reunification" and backed up its warnings by flying regular bomber patrols around it.
Beijing also says Taiwan does not have the right to state-to-state relations and is keen to isolate it diplomatically.
Seven countries have severed diplomatic ties with the Taiwan and switched allegiance to Beijing since Tsai coming Newport Pleasure to power. It now has formal diplomatic ties with just 15 nations.
But Tsai said Taiwan was undaunted.
"The determination of the Taiwanese people to embrace the world has never wavered," she said, adding that Taiwan must work with "like-minded countries" to ensure peace and stability across the Taiwan Strait.
Tsai said under her watch Taiwan has boosted its combat capabilities with the purchase of advanced weapons and development of home-made aircraft.
Taiwan unveiled its largest defense spending increase in more than a decade in August, aiming to purchase more advanced weapons from overseas.
The island has long been a flashpoint in the U.S.-China relationship.In July, the United States approved the sale of an $2.2 billion worth of weapons to Taiwan, angering Beijing.
The United States has no formal ties with Taiwan but is bound by law to help provide it with the Newport 100s Box means to defend itself.